Valery A. Petrenko
A. Petrenko, Professor in the Department of Pathobiology at
Auburn University; graduated
Moscow State University,
U.S.S.R (1972); received PhD and D.Sc. degrees in chemistry from the
Zelinski Institute of Organic
Chemistry (1976) and Moscow State University, U.S.S.R (1988), Honor Ranks of Senior Scientist in Bioorganic Chemistry (1984) and Professor in Molecular Biology
(1992) from the Supreme Attestation Committee of the U.S.S.R. His Ph.D. research
focused on the synthesis of O-specific antigens of Salmonella, and D.Sc.
directed engineering of DNA and proteins. In
1977 he moved to Novosibirsk and worked as Junior and Senior Scientist
(1977-1982), Laboratory Head (1982-1985), Associate Director for Research (1985-1989), Director of Institute,
Vice President for Research and Professor (1989-1993) in
(Novosibirsk, Russia). In 1993 he joined the
faculty of University of
Missouri-Columbia as Visiting Professor and Research Professor, and in
2000 - Auburn University as Professor and Graduate Faculty (2001). In
Auburn Dr. Petrenko established a
research program focused on development of diagnostic and therapeutic probes using
phage display and phage nanobiotechnology. Dr. Petrenko is recipient (PI) of
grants from the Army Research
Institute of Health,
Calvert Research LLC,
and Auburn University, and CoPI in several collaborative grants. He is recipient of the Pfizer Animal Health Awards
for Research Excellence (2006, 2011), Auburn University's Scholarship Incentive
Award (2014), Auburn President's Outstanding Collaborative Units Award in
Pharmaceutical Engineering. He is
National Academy of Inventors Chapter (2013),
Auburn University Research Initiative in Cancer (AURIC), National Cancer
Institute (NCI) Alliance for Nanotechnology in Cancer (2009) and Phi Zeta Honor Society of
Veterinary Medicine, included in
"Who's Who in Medicine and Healthcare", "Who's Who in America" 2011 (65th Edition),
is Who in the World" (15th Edition). and "Who's Who in Science and
Engineering" 2011-2012 (11th Edition).
- Targeted drug/gene delivery
- Diagnosis of infectious diseases and cancer
- Monitoring and detection of biological threats
To attack these
problems, Professor Petrenko employs methods of phage display and phage nanobiotechnology
developed by his group.
The phage model is a gift from Dr.
Gregory P. Kishchenko.
List of Published Work in
MyBibliography (136, h-index ISI: 28) :
2014. Wang T,
Hartner WC, Gillespie JW, Praveen KP, Yang S, Mei LA, Petrenko VA, Torchilin VP.
Enhanced tumor delivery and antitumor activity
in vivo of liposomal doxorubicin
modified with MCF-7-specific phage fusion protein. Nanomedicine. 2014 Feb;10(2).
Wang F, Liu P, Sun L, Li
C, Petrenko VA, Liu A.
Bio-mimetic nanostructure self-assembled from Au@Ag
heterogeneous nanorods and phage fusion proteins for targeted tumor optical
detection and photothermal therapy.
Sci Rep. 2014 Oct 28;4:6808.
Wang T, Yang S, Mei LA,
Parmar CK, Gillespie JW, Praveen KP, Petrenko VA, Torchilin VP.
Paclitaxel-Loaded PEG-PE-Based Micellar
Nanopreparations Targeted with Tumor-Specific Landscape Phage Fusion Protein
Enhance Apoptosis and Efficiently Reduce Tumors.
Mol Cancer Ther. 2014 Dec;13(12):2864-75.
Yin L, Luo Y, Liang B,
Wang F, Du M, Petrenko VA, Qiu HJ, Liu A.
Specific ligands for classical swine fever virus
screened from landscape phage display library. Antiviral Res. 2014
Bedi D, Gillespie JW,
Selection of pancreatic cancer cell-binding
landscape phages and their use in development of anticancer nanomedicines.
Protein Eng Des Sel. 2014 Jul;27(7):235-43.
Qi H, Wang F, Petrenko
VA, Liu A. Peptide microarray with ligands at
high density based on symmetrical carrier landscape phage for detection of
cellulase. Anal Chem. 2014 Jun 17;86(12):5844-50.
Lang Q, Wang F, Yin L, Liu
M, Petrenko VA, Liu A.
Specific probe selection from landscape phage
display library and its application in enzyme-linked immunosorbent assay of free
Anal Chem. 2014 Mar
Petrenko VA, Jayanna PK.
Phage protein-targeted cancer nanomedicines.
FEBS Lett. 2014 Jan 21;588(2):341-9.
Fagbohun OA, Kazmierczak RA, Petrenko VA, Eisenstark A.
Metastatic prostate cancer cell-specific phage-like
particles as a targeted gene-delivery system. J Nanobiotechnology.
2013 Sep 23;11:31.
Knez, Karel; Noppe, Wim; Geukens, Nick;
Janssen, Kris; Spasic, Dragana; Heyligen, Jeroen; Vriens, Kim; Thevissen, Karin
; Cammue, Bruno; Petrenko, Valery; Ulens, Chris; Deckmyn, Hans; Lammertyn,
Jeroen. Affinity comparison of p3 and p8 peptide displaying bacteriophages using
surface plasmon resonance. Analytical Chemistry, 2013, 85, 10075-10082.
Bedi D, Gillespie JW,
Petrenko VA Jr, Ebner A, Leitner M, Hinterdorfer P, Petrenko VA. Targeted
Delivery of siRNA into Breast Cancer Cells via Phage Fusion Proteins.
Mol Pharm. 2013 Feb 4;10(2):551-9.
K. Ma, D.-D. Wang, Y. Lin,
J. Wang, V. Petrenko, C. Mao. Synergetic
Targeted Delivery of Sleeping-Beauty Transposon System to Mesenchymal Stem Cells
Using LPD Nanoparticles Modified with a Phage-Displayed Targeting Peptide.
Adv. Funct. Mater. 2013, 23, 1172–1181.
Samoylova TI, Cochran AM,
Samoylov AM, Schemera B, Breiteneicher AH, Ditchkoff SS, Petrenko VA, Cox
Phage display allows identification of zona
pellucida-binding peptides with species-specific properties: novel approach for
development of contraceptive vaccines for wildlife.
J Biotechnol. 2012 Dec 31;162(2-3):311-8.
Petrenko, V.A.; Torchilin, V.P. Optimization of Landscape Phage Fusion
Protein-Modified Polymeric PEG-PE Micelles for Improved Breast Cancer Cell
Targeting. J Nanomedic Nanotechnol S4:008 (2012).
Huan Qi; Haiqin Lu; Huaji Qiu; Valery A Petrenko; Aihua Liu.Phagemid vectors for phage display: properties, characteristics and construction.
J. Mol. Biol. (2012), 417(3):129-43.
Olusegun A. Fagbohun, Deepa Bedi, Natalia I. Grabchenko,
Patricia A. Deinnocentes, Richard C. Bird, and Valery A. Petrenko. Landscape
phages and their fusion proteins targeted to breast cancer cells. Protein
Engineering, Design & Selection pp. 1–13, 2012.
(Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011)
Valery A. Petrenko and Prashanth K.
Jayanna, Phage-Mediated Drug Delivery. In: Phage Nanobiotechnology (Eds.
Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011).
Suiqiong Li, Ramji S. Lakshmanan, Valery A.
Petrenko and Bryan A. Chin. Chapter 6. Phage-Based Pathogen Biosensors. In:
Phage Nanobiotechnology (Eds. Valery Petrenko & George Smith), RSC Publishing,
Cambridge, UK (2011).
Wang T, Kulkarni N, D'Souza GG, Petrenko
VA, Torchilin VP. On the mechanism of targeting of phage fusion
protein-modified nanocarriers: only the binding Peptide sequence matters.
Mol Pharm. 2011 Oct 3;8(5):1720-8.
Wang T, Kulkarni N, Bedi D, D'Souza GG, Papahadjopoulos-Sternberg B,
Petrenko VA, Torchilin VP. In
vitro optimization of liposomal nanocarriers prepared from breast tumor cell
specific phage fusion protein.
J Drug Target. 2011
Bieke Van Dorst, Jaytry Mehta, Elsa Rouah-Martin, Wim De Coen, Valery Petrenko,
Ronny Blusta and Johan Robbens. Selection of PCB binding phages as potential
biorecognition elements for food and environmental monitoring.
Anal. Methods, 2011, 3, 1865.
Horikawa, S; Bedi, D; Li, SQ; Shen,
W; Huang, SC; Chen, IH; Chai, YT; Auad, ML; Bozack, MJ; Barbaree, JM; Petrenko,
VA; Chin, BA. Effects of surface functionalization on the surface phage coverage
and the subsequent performance of phage-immobilized magnetoelastic biosensors.
Biosensors and Bioelectronics, 2011 Jan 15;26(5):2361-7. Epub 2010 Oct 14.
Deepa Bedi, Tiziana Musacchio,
Olusegun A. Fagbohun, Patricia Deinnocentesa, R. Curtis Bird,
Lonnie Bookbinder, Vladimir
P. Torchilin, and Valery A. Petrenko. Delivery of siRNA into breast cancer cells
via phage fusion protein-targeted liposomes. Nanomedicine: Nanotechnology,
Biology, and Medicine,
315-323 JUN 2011.
2010 Abbineni, Gopal; Modali, Sita; Safiejko-Mroczka, Barbara;
Petrenko, Valery; Mao, Chuanbin. "Evolutionary selection of new breast
cancer cell-targeting peptides and phages with the cell-targeting peptides
fully displayed on the major coat and their effects on actin dynamics during
cell internalization". Mol Pharm. 2010,
VOL. 7, NO. 5, 1629 - 1642. PMID: 20735141
Wang T, Petrenko VA, Torchilin VP.
Polymeric Micelles Modified with MCF-7 Cell-Specific Phage Protein: Enhanced
Binding to Target Cancer Cells and Increased Cytotoxicity.
Pharm. 2010, 7(4):1007-14.PMID: 20518562
Wang T, D'Souza GG, Bedi D, Fagbohun OA,
Potturi LP, Papahadjopoulos-Sternberg B, Petrenko VA, Torchilin VP.
Enhanced binding and
killing of target tumor cells by drug-loaded liposomes modified with
tumor-specific phage fusion coat protein. Nanomedicine (Lond).
2010 Jun;5(4):563-74.PMID: 20528452
Wang T, Yang S, Petrenko VA, Torchilin VP.
of Liposomes into MCF-7 Breast Cancer Cells Mediated by Cell-Specific Phage
Fusion Coat Protein. Mol Pharm. 2010 Jun 14. [Epub ahead of print]PMID:
Jayanna PK, Bedi D, Deinnocentes P, Bird RC, Petrenko VA.
ligands for PC3 prostate carcinoma cells. Protein Eng Des Sel. 2010 Feb
25. [Epub ahead of print]PMID: 20185523
Jayanna PK, Bedi D, Gillespie JW, Deinnocentes P,
Wang T, Torchilin VP, Bird RC, Petrenko VA.
Landscape phage fusion
protein-mediated targeting of nanomedicines enhances their prostate tumor
cell association and cytotoxic efficiency. Nanomedicine. 2010 Feb
4. [Epub ahead of print]
Shen, W; Mathison, LC; Petrenko, VA;
Chin, BA. Design and characterization of a magnetoelastic sensor for the
detection of biological agents. Journal of Physics D-Applied Physics 43 (1):
Art. No. 015004 JAN 13 2010.
2009 G.A. Kuzmicheva, P.K. Jayanna, A.M.
Eroshkin, M.A. Grishina, E.S. Pereyaslavskaya, V.A. Potemkin, and V.A.
Mutations in fd phage major coat protein modulate affinity of the displayed
Protein Engineering, Design and Selection 2009 22: 631-639. PEDS Advance
Access published on July 25, 2009.
Jayanna P.K., Torchilin V.P. and V.A. Petrenko
(2009) Liposomes targeted by fusion phage proteins. Nanomedicine, 2009
Mar;5(1):83-9. Epub 2008 Oct 1.
Shen, W; Lakshmanan, RS; Mathison, LC; Petrenko, VA;
Chin, BA. Phage coated magnetoelastic micro-biosensors for real-time
detection of Bacillus anthracis spores. Sensors and Actuators B-Chemical 137
(2): 501-506 APR 2 2009.
S. Huang, H. Yang, R.S. Lakshmanan, M.L. Johnson, J. Wan, I.-H. Chen,
H.C. Wikle III, V.A. Petrenko, J.M. Barbaree, B.A. Chin. Sequential
detection of Salmonella typhimurium and Bacillus anthracis
sporesusing magnetoelastic biosensors. Biosensors and Bioelectronics.
24 (6), p.1730-1736, Feb 2009.
G.A. Kuzmicheva, P.K. Jayanna, I.B. Sorokulova
and V.A. Petrenko (2009) Diversity and censoring of landscape phage
libraries. Protein Engineering, Design, and Selection. 2009
Jan;22(1):9-18. Epub 2008 Nov 6.
Smith G.P. and Petrenko V.A.
(1997) Phage Display.
Reviews, V.97, N2, p. 391-410.
V.A., Smith G.P.,
Gong X. and Quinn T. (1996) A library of organic landscapes on filamentous
phage. Protein Engineering, V.9, N9, p. 797-801.
Minenkova O.O., A.A. Ilyichev,
G.P. Kishchenko and V.A. Petrenko (1993) Design of specific immunogen
using filamentous phages as a carrier. Gene, 128, 85-88.
Kishchenko G.P., O.O. Minenkova, A.A. Ilyichev, A.D. Gruzdev, V.A. Petrenko (1991) Study of the structure of phage-M13 virions containing chimeric B-protein molecules. Molekulyarnaya biologiya,
25, 1497-1503. Molecular Biology, 1991, 25(6), 1171-1176.
Ilyichev A.A., O.O. Minenkova,
S.I. Tatkov, N.N. Karpyshev, A.M. Eroshkin, V.A. Petrenko, L.S.
Sandakhchiev (1989) Production of a viable variant of phage M13 with
incorporated foreign peptide in the major coat protein. Doklady Akademii
nauk SSSR., 307, 481-483.
Doklady Biochemistry (Proc. Acad. Sci.
USSR)-Engl.Tr., 1989, 307, 196-198.