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Dr. Valery A. Petrenko

Valery A. Petrenko, Professor in the Department of Pathobiology at Auburn University; graduated from Moscow State University, U.S.S.R (1972); received PhD and D.Sc. degrees in chemistry from the Zelinski Institute of Organic Chemistry (1976) and Moscow State University, U.S.S.R (1988), Honor Ranks of Senior Scientist in Bioorganic Chemistry (1984) and Professor in Molecular Biology (1992) from the Supreme Attestation Committee of the U.S.S.R. His Ph.D. research focused on the synthesis of O-specific antigens of Salmonella, and D.Sc. - on directed engineering of DNA and proteins. In 1977 he moved to Novosibirsk and worked as Junior and Senior Scientist (1977-1982), Laboratory Head (1982-1985), Associate Director for Research (1985-1989), Director of Institute, Vice President for Research and Professor (1989-1993) in Scientific Association Vector (Novosibirsk, Russia). In 1993 he joined the faculty of University of Missouri-Columbia as Visiting Professor and Research Professor, and in 2000 - Auburn University as Professor and Graduate Faculty (2001). In Auburn, Dr. Petrenko established a research program focused on development of diagnostic and therapeutic probes using phage display and phage nanobiotechnology. Dr. Petrenko is recipient (PI) of grants from the Army Research Office, the National Institute of Health, Calvert Research, LLC, and Auburn University, and serves as Co-PI in several collaborative grants. He is recipient of the Pfizer Animal Health Awards for Research Excellence (2006, 2011), Auburn University's Scholarship Incentive Award (2014), Auburn President's Outstanding Collaborative Units Award in Pharmaceutical Engineering. He is member of National Academy of Inventors Chapter (2013), Auburn University Research Initiative in Cancer (AURIC), National Cancer Institute (NCI) Alliance for Nanotechnology in Cancer (2009) and Phi Zeta Honor Society of Veterinary Medicine, included in "Who's Who in Medicine and Healthcare", "Who's Who in America" 2011 (65th Edition), "Who is Who in the World" (15th Edition) and "Who's Who in Science and Engineering" 2011-2012 (11th Edition).

The phage model is a gift from Dr. Gregory P. Kishchenko.

Research Interests

  • Targeted Drug/Gene Delivery
  • Diagnosis of Infectious Diseases and Cancer
  • Monitoring and Detection of Biological Threats

To attack these problems, Professor Petrenko employs methods of phage display and phage nanobiotechnology developed by his group.


List of Published Work in My NCBI Bibliography (136, h-index ISI: 28): http://www.ncbi.nlm.nih.gov/sites/myncbi/valery.petrenko.1/bibliography/41152535/public/

Recent Publications


  • Gross AL, Gillespie JW, and Petrenko VA. Promiscuous tumor targeting phage proteins. Protein Eng Des Sel. 2016 Feb; 29(3):93-103.


  • Gillespie JW, Gross AL, Puzyrev AT, Bedi D, and Petrenko VA. Combinatorial synthesis and screening of cancer cell-specific nanomedicines targeted via phage fusion proteins. Frontiers in Microbiology. 2015 Jun; 6:628.


  • Wang T, Hartner WC, Gillespie JW, Praveen KP, Yang S, Mei LA, Petrenko VA, Torchilin VP. Enhanced tumor delivery and antitumor activity in vivo of liposomal doxorubicin modified with MCF-7-specific phage fusion protein. Nanomedicine. 2014 Feb; 10(2).
  • Wang F, Liu P, Sun L, Li C, Petrenko VA, Liu A. Bio-mimetic nanostructure self-assembled from Au@Ag heterogeneous nanorods and phage fusion proteins for targeted tumor optical detection and photothermal therapy. Sci Rep. 2014 Oct 28;4:6808.
  • Wang T, Yang S, Mei LA, Parmar CK, Gillespie JW, Praveen KP, Petrenko VA, Torchilin VP. Paclitaxel-Loaded PEG-PE-Based Micellar Nanopreparations Targeted with Tumor-Specific Landscape Phage Fusion Protein Enhance Apoptosis and Efficiently Reduce Tumors. Mol Cancer Ther. 2014 Dec;13(12):2864-75.
  • Yin L, Luo Y, Liang B, Wang F, Du M, Petrenko VA, Qiu HJ, Liu A. Specific ligands for classical swine fever virus screened from landscape phage display library. Antiviral Res. 2014 Sep;109:68-71.
  • Bedi D, Gillespie JW, Petrenko VA. Selection of pancreatic cancer cell-binding landscape phages and their use in development of anticancer nanomedicines. Protein Eng Des Sel. 2014 Jul;27(7):235-43.
  • Qi H, Wang F, Petrenko VA, Liu A. Peptide microarray with ligands at high density based on symmetrical carrier landscape phage for detection of cellulase. Anal Chem. 2014 Jun 17;86(12):5844-50.
  • Lang Q, Wang F, Yin L, Liu M, Petrenko VA, Liu A. Specific probe selection from landscape phage display library and its application in enzyme-linked immunosorbent assay of free prostate-specific antigen. Anal Chem. 2014 Mar 4;86(5):2767-74.
  • Petrenko VA, Jayanna PK. Phage protein-targeted cancer nanomedicines. FEBS Lett. 2014 Jan 21;588(2):341-9.


  • Fagbohun OA, Kazmierczak RA, Petrenko VA, Eisenstark A. Metastatic prostate cancer cell-specific phage-like particles as a targeted gene-delivery system. J Nanobiotechnology. 2013 Sep 23;11:31.
  • Knez, Karel; Noppe, Wim; Geukens, Nick; Janssen, Kris; Spasic, Dragana; Heyligen, Jeroen; Vriens, Kim; Thevissen, Karin; Cammue, Bruno; Petrenko, Valery; Ulens, Chris; Deckmyn, Hans; Lammertyn, Jeroen. Affinity comparison of p3 and p8 peptide displaying bacteriophages using surface plasmon resonance. Analytical Chemistry, 2013, 85, 10075-10082.
  • Bedi D, Gillespie JW, Petrenko VA Jr, Ebner A, Leitner M, Hinterdorfer P, Petrenko VA. Targeted Delivery of siRNA into Breast Cancer Cells via Phage Fusion Proteins. Mol Pharm. 2013 Feb 4;10(2):551-9.
  • K. Ma, D.-D. Wang, Y. Lin, J. Wang, V. Petrenko, C. Mao. Synergetic Targeted Delivery of Sleeping-Beauty Transposon System to Mesenchymal Stem Cells Using LPD Nanoparticles Modified with a Phage-Displayed Targeting Peptide. Adv. Funct. Mater. 2013, 23, 1172-1181.


  • Samoylova TI, Cochran AM, Samoylov AM, Schemera B, Breiteneicher AH, Ditchkoff SS, Petrenko VA, Cox NR. Phage display allows identification of zona pellucida-binding peptides with species-specific properties: novel approach for development of contraceptive vaccines for wildlife. J Biotechnol. 2012 Dec 31;162(2-3):311-8.
  • Wang, T.; Petrenko, V.A.; Torchilin, V.P. Optimization of Landscape Phage Fusion Protein-Modified Polymeric PEG-PE Micelles for Improved Breast Cancer Cell Targeting. J Nanomedic Nanotechnol S4:008 (2012).
  • Huan Qi; Haiqin Lu; Huaji Qiu; Valery A Petrenko; Aihua Liu. Phagemid vectors for phage display: properties, characteristics and construction. J. Mol. Biol. (2012), 417(3):129-43.
  • Olusegun A. Fagbohun, Deepa Bedi, Natalia I. Grabchenko, Patricia A. Deinnocentes, Richard C. Bird, and Valery A. Petrenko. Landscape phages and their fusion proteins targeted to breast cancer cells. Protein Engineering, Design & Selection pp. 1-13, 2012.


  • Phage Nanobiotechnology (Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011)
  • Valery A. Petrenko and Prashanth K. Jayanna, Phage-Mediated Drug Delivery. In: Phage Nanobiotechnology (Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011).
  • Suiqiong Li, Ramji S. Lakshmanan, Valery A. Petrenko and Bryan A. Chin. Chapter 6. Phage-Based Pathogen Biosensors. In: Phage Nanobiotechnology (Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011).
  • Wang T, Kulkarni N, D'Souza GG, Petrenko VA, Torchilin VP. On the mechanism of targeting of phage fusion protein-modified nanocarriers: only the binding Peptide sequence matters. Mol Pharm. 2011 Oct;8(5):1720-8.
  • Wang T, Kulkarni N, Bedi D, D'Souza GG, Papahadjopoulos-Sternberg B, Petrenko VA, Torchilin VP. In vitro optimization of liposomal nanocarriers prepared from breast tumor cell specific phage fusion protein. J Drug Target. 2011 Sep;19(8):597-605.
  • Bieke Van Dorst, Jaytry Mehta, Elsa Rouah-Martin, Wim De Coen, Valery Petrenko, Ronny Blusta and Johan Robbens. Selection of PCB binding phages as potential biorecognition elements for food and environmental monitoring. Anal. Methods, 2011, 3, 1865.
  • Horikawa, S; Bedi, D; Li, SQ; Shen, W; Huang, SC; Chen, IH; Chai, YT; Auad, ML; Bozack, MJ; Barbaree, JM; Petrenko, VA; Chin, BA. Effects of surface functionalization on the surface phage coverage and the subsequent performance of phage-immobilized magnetoelastic biosensors. Biosensors and Bioelectronics, 2011 Jan 15;26(5):2361-7. Epub 2010 Oct 14.
  • Deepa Bedi, Tiziana Musacchio, Olusegun A. Fagbohun, Patricia Deinnocentesa, R. Curtis Bird, Lonnie Bookbinder, Vladimir P. Torchilin, and Valery A. Petrenko. Delivery of siRNA into breast cancer cells via phage fusion protein-targeted liposomes. Nanomedicine: Nanotechnology, Biology, and Medicine, 7 (3):315-323 JUN 2011.


  • Abbineni, Gopal; Modali, Sita; Safiejko-Mroczka, Barbara; Petrenko, Valery; Mao, Chuanbin. Evolutionary selection of new breast cancer cell-targeting peptides and phages with the cell-targeting peptides fully displayed on the major coat and their effects on actin dynamics during cell internalization. Mol Pharm. 2010, VOL. 7, NO. 5, 1629 - 1642. PMID: 20735141
  • Wang T, Petrenko VA, Torchilin VP. Paclitaxel-Loaded Polymeric Micelles Modified with MCF-7 Cell-Specific Phage Protein: Enhanced Binding to Target Cancer Cells and Increased Cytotoxicity. Mol. Pharm. 2010, 7(4):1007-14.PMID: 20518562
  • Wang T, D'Souza GG, Bedi D, Fagbohun OA, Potturi LP, Papahadjopoulos-Sternberg B, Petrenko VA, Torchilin VP. Enhanced binding and killing of target tumor cells by drug-loaded liposomes modified with tumor-specific phage fusion coat protein. Nanomedicine (Lond). 2010 Jun;5(4):563-74.PMID: 20528452
  • Wang T, Yang S, Petrenko VA, Torchilin VP. Cytoplasmic Delivery of Liposomes into MCF-7 Breast Cancer Cells Mediated by Cell-Specific Phage Fusion Coat Protein. Mol Pharm. 2010 Jun 14. [Epub ahead of print]PMID: 20438086
  • Jayanna PK, Bedi D, Deinnocentes P, Bird RC, Petrenko VA. Landscape phage ligands for PC3 prostate carcinoma cells. Protein Eng Des Sel. 2010 Feb 25. [Epub ahead of print]PMID: 20185523
  • Jayanna PK, Bedi D, Gillespie JW, Deinnocentes P, Wang T, Torchilin VP, Bird RC, Petrenko VA. Landscape phage fusion protein-mediated targeting of nanomedicines enhances their prostate tumor cell association and cytotoxic efficiency. Nanomedicine. 2010 Feb 4. [Epub ahead of print]
  • Shen, W; Mathison, LC; Petrenko, VA; Chin, BA. Design and characterization of a magnetoelastic sensor for the detection of biological agents. Journal of Physics D-Applied Physics 43 (1): Art. No. 015004 JAN 13 2010.


  • G.A. Kuzmicheva, P.K. Jayanna, A.M. Eroshkin, M.A. Grishina, E.S. Pereyaslavskaya, V.A. Potemkin, and V.A. Petrenko (2009) Mutations in fd phage major coat protein modulate affinity of the displayed peptide. Protein Engineering, Design and Selection 2009 22: 631-639. PEDS Advance Access published on July 25, 2009.
  • Jayanna P.K., Torchilin V.P. and V.A. Petrenko (2009) Liposomes targeted by fusion phage proteins. Nanomedicine, 2009 Mar;5(1):83-9. Epub 2008 Oct 1.
  • Shen, W; Lakshmanan, RS; Mathison, LC; Petrenko, VA; Chin, BA. Phage coated magnetoelastic micro-biosensors for real-time detection of Bacillus anthracis spores. Sensors and Actuators B-Chemical 137 (2): 501-506 APR 2 2009.
  • S. Huang, H. Yang, R.S. Lakshmanan, M.L. Johnson, J. Wan, I.-H. Chen, H.C. Wikle III, V.A. Petrenko, J.M. Barbaree, B.A. Chin. Sequential detection of Salmonella typhimurium and Bacillus anthracis spores using magnetoelastic biosensors. Biosensors and Bioelectronics. 24 (6), p.1730-1736, Feb 2009.
  • G.A. Kuzmicheva, P.K. Jayanna, I.B. Sorokulova and V.A. Petrenko Diversity and censoring of landscape phage libraries. Protein Engineering, Design, and Selection. 2009 Jan;22(1):9-18. Epub 2008 Nov 6.

Earlier Publications

  • Smith G.P. and Petrenko V.A. (1997) Phage Display. Chemical Reviews, V.97, N2, p. 391-410.
  • Petrenko V.A., Smith G.P., Gong X. and Quinn T. (1996) A library of organic landscapes on filamentous phage. Protein Engineering, V.9, N9, p. 797-801.
  • Minenkova O.O., A.A. Ilyichev, G.P. Kishchenko and V.A. Petrenko (1993) Design of specific immunogen using filamentous phages as a carrier. Gene, 128, 85-88.
  • Kishchenko G.P., O.O. Minenkova, A.A. Ilyichev, A.D. Gruzdev, V.A. Petrenko (1991) Study of the structure of phage-M13 virions containing chimeric B-protein molecules. Molekulyarnaya biologiya, 25, 1497-1503. Molecular Biology, 1991, 25(6), 1171-1176.
  • Ilyichev A.A., O.O. Minenkova, S.I. Tatkov, N.N. Karpyshev, A.M. Eroshkin, V.A. Petrenko, L.S. Sandakhchiev (1989) Production of a viable variant of phage M13 with incorporated foreign peptide in the major coat protein.Doklady Akademiinauk SSSR., 307, 481-483. Doklady Biochemistry (Proc. Acad. Sci. USSR)-Engl.Tr., 1989, 307, 196-198.
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