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Dr. Valery A. Petrenko

Valery A. Petrenko, Professor in the Department of Pathobiology at Auburn University; graduated from Moscow State University, U.S.S.R (1972); received PhD and D.Sc. degrees in chemistry from the Zelinski Institute of Organic Chemistry, (1976) and Moscow State University, U.S.S.R (1988), Honor Ranks of Senior Scientist in Bioorganic Chemistry (1984) and Professor in Molecular Biology (1992) from the Supreme Attestation Committee of the Government of the U.S.S.R. His Ph.D. research focused on the synthesis of O-specific antigens of Salmonella, and D.Sc.—on directed engineering of DNA and proteins.  In 1977 he moved to Novosibirsk and worked as Junior and Senior Scientist (1977-1982), Laboratory Head (1982-1985), Associate Director for Science (1985-1989), Director of Institute, Vice President for Science and Professor (1989-1993) in Scientific Association “Vector” (Novosibirsk, Russia).  In 1993 he joined the faculty of University of Missouri-Columbia as Visiting Professor and Research Professor, and in 2000—Auburn University as Professor. He was appointed to the Graduate Faculty in 2001. In Auburn Dr. Petrenko established a research program focused on development of diagnostic and therapeutic probes using phage display and phage nanobiotechnology. Dr. Petrenko is recipient (PI) of grants from the Army Research Office and the National Institute of Health, Calvert Research LLC, recipient of the Pfizer Animal Health Award for Research Excellence (2006, 2011), Auburn University's Scholarship Incentive Award (2014), Auburn President's Outstanding Collaborative Units Award in Pharmaceutical Engineering and CoPI in several collaborative grants. He is member of National Academy of Inventors Chapter (2013), Auburn University Research Initiative in Cancer (AURIC), National Cancer Institute (NCI) Alliance for Nanotechnology in Cancer (2009) and Phi Zeta Honor Society of Veterinary Medicine, included in "Who's Who in Medicine and Healthcare", "Who's Who in America" 2011 (65th Edition), "Who is Who in the World" (15th Edition). and "Who's Who in Science and Engineering" 2011-2012 (11th Edition). .

Research Interests

  • Targeted drug/gene delivery
  • Diagnosis of infectious diseases and cancer
  • Monitoring and detection of biological threats 

To attack these problems, Professor Petrenko employs methods of phage display and phage nanobiotechnology developed by his group.


The phage model is a gift from Dr. Gregory P. Kishchenko.

Selected Publications

2014. Wang T, Hartner WC, Gillespie JW, Praveen KP, Yang S, Mei LA, Petrenko VA, Torchilin VP. Enhanced tumor delivery and antitumor activity in vivo of liposomal doxorubicin modified with MCF-7-specific phage fusion protein. Nanomedicine. 2014 Feb;10(2).

Wang F, Liu P, Sun L, Li C, Petrenko VA, Liu A. Bio-mimetic nanostructure self-assembled from Au@Ag heterogeneous nanorods and phage fusion proteins for targeted tumor optical detection and photothermal therapy.  Sci Rep. 2014 Oct 28;4:6808.

Wang T, Yang S, Mei LA, Parmar CK, Gillespie JW, Praveen KP, Petrenko VA, Torchilin VP. Paclitaxel-Loaded PEG-PE-Based Micellar Nanopreparations Targeted with Tumor-Specific Landscape Phage Fusion Protein Enhance Apoptosis and Efficiently Reduce Tumors. Mol Cancer Ther. 2014 Dec;13(12):2864-75. 

Yin L, Luo Y, Liang B, Wang F, Du M, Petrenko VA, Qiu HJ, Liu A. Specific ligands for classical swine fever virus screened from landscape phage display library. Antiviral Res. 2014 Sep;109:68-71.

Bedi D, Gillespie JW, Petrenko VA. Selection of pancreatic cancer cell-binding landscape phages and their use in development of anticancer nanomedicines. Protein Eng Des Sel. 2014 Jul;27(7):235-43.

Qi H, Wang F, Petrenko VA, Liu A. Peptide microarray with ligands at high density based on symmetrical carrier landscape phage for detection of cellulase. Anal Chem. 2014 Jun 17;86(12):5844-50.

Lang Q, Wang F, Yin L, Liu M, Petrenko VA, Liu A. Specific probe selection from landscape phage display library and its application in enzyme-linked immunosorbent assay of free prostate-specific antigen. Anal Chem. 2014 Mar 4;86(5):2767-74.

Petrenko VA, Jayanna PK. Phage protein-targeted cancer nanomedicines. FEBS Lett. 2014 Jan 21;588(2):341-9.

2013.  V.A. Petrenko and P.K. Jayanna. Phage protein-targeted cancer nanomedicines. FEBS Lett. (2013), http://dx.doi.org/ http://dx.doi.org/10.1016/j.febslet.2013.11.011

Fagbohun OA, Kazmierczak RA, Petrenko VA, Eisenstark A. Metastatic prostate cancer cell-specific phage-like particles as a targeted gene-delivery system. J Nanobiotechnology. 2013 Sep 23;11:31. 

Knez, Karel; Noppe, Wim; Geukens, Nick; Janssen, Kris; Spasic, Dragana; Heyligen, Jeroen; Vriens, Kim; Thevissen, Karin ; Cammue, Bruno; Petrenko, Valery; Ulens, Chris; Deckmyn, Hans; Lammertyn, Jeroen. Affinity comparison of p3 and p8 peptide displaying bacteriophages using surface plasmon resonance. Analytical Chemistry, 2013, 85, 10075-10082.

Bedi D, Gillespie JW, Petrenko VA Jr, Ebner A, Leitner M, Hinterdorfer P, Petrenko VA. Targeted Delivery of siRNA into Breast Cancer Cells via Phage Fusion Proteins. Mol Pharm. 2013 Feb 4;10(2):551-9.

K. Ma, D.-D. Wang, Y. Lin, J. Wang, V. Petrenko, C. Mao. Synergetic Targeted Delivery of Sleeping-Beauty Transposon System to Mesenchymal Stem Cells Using LPD Nanoparticles Modified with a Phage-Displayed Targeting Peptide. Adv. Funct. Mater. 2013, 23, 1172–1181.

Samoylova TI, Cochran AM, Samoylov AM, Schemera B, Breiteneicher AH, Ditchkoff SS, Petrenko VA, Cox NR. Phage display allows identification of zona pellucida-binding peptides with species-specific properties: novel approach for development of contraceptive vaccines for wildlife. J Biotechnol. 2012 Dec 31;162(2-3):311-8.

2012.  Wang, T.; Petrenko, V.A.; Torchilin, V.P. Optimization of Landscape Phage Fusion Protein-Modified Polymeric PEG-PE Micelles for Improved Breast Cancer Cell Targeting. J Nanomedic Nanotechnol S4:008 (2012).

Huan Qi; Haiqin Lu; Huaji Qiu; Valery A Petrenko; Aihua Liu.Phagemid vectors for phage display: properties, characteristics and construction. J. Mol. Biol. (2012), 417(3):129-43.

Olusegun A. Fagbohun, Deepa Bedi, Natalia I. Grabchenko, Patricia A. Deinnocentes, Richard C. Bird, and Valery A. Petrenko. Landscape phages and their fusion proteins targeted to breast cancer cells. Protein Engineering, Design & Selection pp. 1–13, 2012.

2011. Phage Nanobiotechnology (Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011)

Valery A. Petrenko  and Prashanth K. Jayanna, Phage-Mediated Drug Delivery. In: “Phage Nanobiotechnology” (Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011).

Suiqiong Li, Ramji S. Lakshmanan, Valery A. Petrenko and Bryan A. Chin. Chapter 6.  Phage-Based Pathogen Biosensors. In: “Phage Nanobiotechnology” (Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011).

Wang T, Kulkarni N, D'Souza GG, Petrenko VA, Torchilin VP. On the mechanism of targeting of phage fusion protein-modified nanocarriers: only the binding Peptide sequence matters. Mol Pharm. 2011 Oct 3;8(5):1720-8.

Wang T, Kulkarni N, Bedi D, D'Souza GG, Papahadjopoulos-Sternberg B, Petrenko VA, Torchilin VP. In vitro optimization of liposomal nanocarriers prepared from breast tumor cell specific phage fusion protein. J Drug Target. 2011 Sep;19(8):597-605.

Bieke Van Dorst, Jaytry Mehta, Elsa Rouah-Martin, Wim De Coen, Valery Petrenko, Ronny Blusta and Johan Robbens. Selection of PCB binding phages as potential biorecognition elements for food and environmental monitoring.  Anal. Methods, 2011, 3, 1865.

Horikawa, S; Bedi, D; Li, SQ; Shen, W; Huang, SC; Chen, IH; Chai, YT; Auad, ML; Bozack, MJ; Barbaree, JM; Petrenko, VA; Chin, BA. Effects of surface functionalization on the surface phage coverage and the subsequent performance of phage-immobilized magnetoelastic biosensors. Biosensors and Bioelectronics,  2011 Jan 15;26(5):2361-7. Epub 2010 Oct 14.

Deepa Bedi,  Tiziana Musacchio, Olusegun A. Fagbohun, Patricia Deinnocentesa, R. Curtis Bird,  Lonnie Bookbinder,  Vladimir P. Torchilin, and Valery A. Petrenko. Delivery of siRNA into breast cancer cells via phage fusion protein-targeted liposomes. Nanomedicine: Nanotechnology, Biology, and Medicine, 7 (3): 315-323 JUN 2011.

2010 Abbineni, Gopal; Modali, Sita; Safiejko-Mroczka, Barbara; Petrenko, Valery; Mao, Chuanbin. "Evolutionary selection of new breast cancer cell-targeting peptides and phages with the cell-targeting peptides fully displayed on the major coat and their effects on actin dynamics during cell internalization". Mol Pharm. 2010, VOL. 7, NO. 5, 1629–1642. PMID: 20735141

Wang T, Petrenko VA, Torchilin VP. Paclitaxel-Loaded Polymeric Micelles Modified with MCF-7 Cell-Specific Phage Protein: Enhanced Binding to Target Cancer Cells and Increased Cytotoxicity. Mol Pharm. 2010, 7(4):1007-14.PMID: 20518562

Wang T, D'Souza GG, Bedi D, Fagbohun OA, Potturi LP, Papahadjopoulos-Sternberg B, Petrenko VA, Torchilin VP. Enhanced binding and killing of target tumor cells by drug-loaded liposomes modified with tumor-specific phage fusion coat protein.  Nanomedicine (Lond). 2010 Jun;5(4):563-74.PMID: 20528452

Wang T, Yang S, Petrenko VA, Torchilin VP. Cytoplasmic Delivery of Liposomes into MCF-7 Breast Cancer Cells Mediated by Cell-Specific Phage Fusion Coat Protein. Mol Pharm. 2010 Jun 14. [Epub ahead of print]PMID: 20438086

Jayanna PK, Bedi D, Deinnocentes P, Bird RC, Petrenko VA. Landscape phage ligands for PC3 prostate carcinoma cells. Protein Eng Des Sel. 2010 Feb 25. [Epub ahead of print]PMID: 20185523

 Jayanna PK, Bedi D, Gillespie JW, Deinnocentes P, Wang T, Torchilin VP, Bird RC, Petrenko VA. Landscape phage fusion protein-mediated targeting of nanomedicines enhances their prostate tumor cell association and cytotoxic efficiency.  Nanomedicine. 2010 Feb 4. [Epub ahead of print]

Shen, W; Mathison, LC; Petrenko, VA; Chin, BA. Design and characterization of a magnetoelastic sensor for the detection of biological agents. Journal of Physics D-Applied Physics 43 (1): Art. No. 015004 JAN 13 2010.

2009  G.A. Kuzmicheva, P.K. Jayanna, A.M. Eroshkin, M.A. Grishina, E.S. Pereyaslavskaya, V.A. Potemkin, and V.A. Petrenko (2009) Mutations in fd phage major coat protein modulate affinity of the displayed peptide. Protein Engineering, Design and Selection 2009 22: 631-639. PEDS Advance Access published on July 25, 2009.

Jayanna P.K., Torchilin V.P. and V.A. Petrenko (2009) Liposomes targeted by fusion phage proteins. Nanomedicine,  2009 Mar;5(1):83-9. Epub 2008 Oct 1.

Shen, W; Lakshmanan, RS; Mathison, LC; Petrenko, VA; Chin, BA. Phage coated magnetoelastic micro-biosensors for real-time detection of Bacillus anthracis spores. Sensors and Actuators B-Chemical 137 (2): 501-506 APR 2 2009.

S. Huang, H. Yang, R.S. Lakshmanan, M.L. Johnson, J. Wan, I.-H. Chen, H.C. Wikle III, V.A. Petrenko, J.M. Barbaree, B.A. Chin. Sequential detection of Salmonella typhimurium and Bacillus anthracis sporesusing magnetoelastic biosensors. Biosensors and Bioelectronics. 24 (6), p.1730-1736, Feb 2009.

G.A. Kuzmicheva, P.K. Jayanna, I.B. Sorokulova and V.A. Petrenko (2009) Diversity and censoring of landscape phage libraries. Protein Engineering, Design, and Selection. 2009 Jan;22(1):9-18. Epub 2008 Nov 6.

2008  S. Huang, S.-Q. Li, H. Yang, M. Johnson, J. Wan, I. Chen, V. A. Petrenko, J. M. Barbaree, and B. A. Chin. Optimization of Phage-Based Magnetoelastic Biosensor Performance. Sensors & Transducers Journal, Vol. 3, Special Issue, December 2008, pp.87-96.  Available online: http://www.sensorsportal.com/HTML/DIGEST/P_SI_59.htm

Petrenko V.A.  (2008) Evolution of Phage Display: From Bioactive Peptides to Bioselective Nanomaterials. Expert Opinion on Drug Delivery (EODD), Aug;5(8):825-36.

S. Huang, H. Yang, R.S. Lakshmanan, M.L. Johnson, I. Chen, J. Wan, H.C. Wikle, V.A. Petrenko, J.M. Barbaree, Z.Y. Cheng, B.A. Chin (2008). The Effect of Salt and Phage Concentrations on the Binding Sensitivity of Magnetoelastic Biosensors for Bacillus anthracis Detection. Biotechnology and Bioengineering, 101 (5): 1014-1021 DEC 1 2008.

Johnson M.L., Wan J., Huang S., Cheng Z., Petrenko V.A. Kim D.-J., Chen I.-H., Barbaree J.M.,  Hong J.W., and B.A. Chin (2008). A Wireless Biosensor Using Microfabricated Phage-Interfaced Magnetoelastic Particles. Sensors & Actuators A: Physical. Vol 144/1 pp 38-47. 

Brigati, J.R., Samoylova, T.I., Jayanna, P.K. and V.A. Petrenko (2008) Phage display technique for generating peptide reagents. In: Current Protocols in Protein Science (Editor Ben M. Dunn, et al.). John Wiley & Sons.

Petrenko V.A.  (2008). Landscape phage as a molecular recognition interface for detection devices.  Review. Microelectronics Journal,  Vol 39/2 pp 202-207. 

Suiqiong Li, Michael Johnson, Jiehui Wan, Valery Petrenko, and Bryan Chin. “Microfabricated Magnetoelastic Biosensors for the Detection of Bacillus Anthracis Spores.” ECS Transactions (Issue Title: Chemical Sensors 8: Chemical (Gas, Ion, Bio) Sensors and Analytical Systems), Vol. 16, pp 177-185, 2008. doi:10.1149/1.2981119

2007  Fu. L., Li, S., Zhang, K., Chen, I-H., Petrenko, V.A.  and Z.-Y. Cheng, (2007), Magnetostrictive microcantilever as an advanced transducer for biosensors, Sensors Journal, V.7, 2929-2941.

Lakshmanan, RS; Guntupalli, R; Hu, J; Petrenko, VA; Barbaree, JM; Chin, BA. Detection of Salmonella typhimurium in fat free milk using a phage immobilized magnetoelastic sensor. Sensors and actuators B-Chemical 126 (2): 544-550 OCT 1 2007

Lakshmanan RS, Guntupalli R, Hu J, Kim DJ, Petrenko VA, Barbaree JM, Chin BA (2007). Phage based magnetoelastic sensor for the detection of Salmonella typhimurium. Microbiol Methods. Oct;71(1):55-60. Epub 2007 Aug 9.

Valery A. Petrenko (2007) Phage-Derived Bioselective Nanovehicles For Drug And Gene Delivery. Nanotech 2007, V. 2. p.703-706, Technical Proceedings, CRC Press, Taylor & Francis Group.

Wan, J., Johnson, M.,  Guntupalli,R.,  Petrenko,V.A. and B.A. Chin (2007), "Detection of Bacillus anthracis spores in liquid using phage-based magnetoelastic micro-resonators", Sensors and actuators B, 127: 559-566.

Wan, J., Shu, H., Huang, S., Fiebor, B., Chen, I-H., Petrenko, V.A., Chin, B.A. (2007), Phage-based Magnetoelastic wireless biosensors for detecting Bacillus anthracis spores. IEEE Sensor Journal, vol 7 No. 3 pp 470-477.

Petrenko V.A. &  J.R. Brigati (2007) Phage As Biospecific Probes. In: Immunoassay and other Bioanalytical Techniques (Editor Jeanette M. Van Emon). CRC Press, Taylor & Francis Group, Boca Raton, FL, U.S.A..

Nanduri V., Sorokulova I.B., Samoylov A.M., Simonian A.L., Petrenko V.A., and V.J. Vodyanoy (2007).  Phage as molecular recognition elements in biosensors immobilized by physical adsorption. Biosensors and Bioelectronics. 22(6):986-92..

E.Olsen, J. Sykora, I. Sorokulova, W. Neely, I. Chen, J. Barbaree V. Petrenko and V. Vodyanoy (2007). Phage Fusion Proteins as Bioselective Receptors for Piezoelectric Sensors. ECS Transactions, 2(19), 9-25.  

2006  Olsen E.V., Sorokulova I.B., Petrenko V.A., Chen I-H., Barbaree J.M., and V.J. Vodyanoy (2006).  Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium.  Biosensors and Bioelectronics. V21: p.1434-1442.

2005  Petrenko V.A. & G.P. Smith (2005) Vectors and Modes of Display. In: Phage Display in Biotechnology and Drug Discovery (Editor S. Sidhu). CRC Press, Taylor & Francis Group, Boca Raton, FL, U.S.A., 714pp.

Brigati J.R. and V.A. Petrenko (2005).  Thermostability of Landscape Phage Probes.  Analytical and Bioanalytical Chemistry. V.382, p.1346-1350.

Sorokulova I.B., E.V. Olsen, I-H. Chen, B. Fiebor, J.M. Barbaree, V.J. Vodyanoy, B.A. Chin and V.A. Petrenko (2005).  Landscape Phage Probes for Salmonella typhimurium.   The Journal of Microbiological Methods. V. 63, No 1, p. 55-72..

2004  Mount J., T.I. Samoylova, N.E. Morrison, N.R. Cox, H.J. Baker and V.A. Petrenko. (2004) Cell Targeted Phagemid Recurred by Pre-Selected Landscape Phage. Gene. V.341, p.59-65.

Brigati J., D.D. Williams, I.B. Sorokulova, V. Nanduri, I-H. Chen, C.L. Turnbough, Jr. and V.A. Petrenko (2004).  Diagnostic probes for Bacillus anthracis spores selected from a landscape phage library.  Clinical Chemistry. V.50, No.11, p.1899-1906.

Samoylova T.I. , N.R. Cox, N.E. Morrison, L.P. Globa, V. Romanov, H.J. Baker, and V.A. Petrenko (2004).  Phage matrix for isolation of glioma cell-membrane proteins.  BioTechniques, V.37, No. 2, p.254-260.


Petrenko V.A. and I.B. Sorokulova (2004) Detection of Biological Threat Agents. A challenge for Combinatorial Biochemistry. The Journal of Microbiological Methods Vol 58/2 pp 147-168.


2003  Samoylova T.I., V.A. Petrenko, N.E. Morrison, L.P. Globa, H.J. Baker, and N.R. Cox (2003). Phage Probes for Malignant Glial Cells.  Molecular Cancer Therapeutic.   V.2, p.1129-1137.


Petrenko V.A. and V.J. Vodyanoy (2003) Phage display for detection of biological threat agents.  The Journal of Microbiological Methods, 53/2 pp. 243-252. 


2002  Petrenko V.A., Smith G.P., Mazooji M.M. and Quinn T.  (2002) Alfa-helically constrained phage display library.  Protein Engineering, V.15, No.11, pp.943-950. 


2001  Romanov V.I., Durand D.E. and Petrenko V.A. (2001) Phage Display Selection On Prostate Carcinoma Cells Yields A Peptide Affecting Cells Spreading And Invasion.  The Prostate, V.47, p.239-251. 

Kouzmitcheva G.A., Petrenko V.A. and Smith G.P. (2001) Diagnostic Peptides for Lyme Disease Through Epitope Discovery. Clinical and  Diagnostic Laboratory Immunology, V8, p.150-160.


2000  Petrenko V.A. and Smith G.P. (2000) Phage from landscape libraries as substitute antibodies.   Protein Engineering, V.13, N8, p.101-104.

1989-1998   Smith G.P., V.A. Petrenko and L.L. Metthews (1998) Cross-Linked Filamentous Phage as an Affinity Matrix. Journal of Immunological Methods, V.215, p.151-161.


Principal Earlier Papers. Smith G.P. and Petrenko V.A. (1997) Phage Display. Chemical Reviews, V.97, N2, p. 391-410. 


Petrenko V.A., Smith G.P., Gong X. and Quinn T. (1996) A library of organic landscapes on filamentous phage. Protein Engineering, V.9, N9, p. 797-801.

Minenkova O.O., A.A. Ilyichev, G.P. Kishchenko and V.A. Petrenko (1993) Design  of  specific  immunogen  using  filamentous phages as a carrier. Gene, 128, 85-88.


Kishchenko G.P., O.O. Minenkova, A.A. Ilyichev, A.D. Gruzdev, V.A. Petrenko (1991) Study of the structure of phage-M13 virions containing chimeric B-protein molecules. Molekulyarnaya biologiya, 25, 1497-1503. Molecular Biology, 1991, 25(6), 1171-1176.


Ilyichev A.A., O.O. Minenkova, S.I. Tatkov, N.N. Karpyshev, A.M. Eroshkin, V.A. Petrenko,  L.S.  Sandakhchiev (1989) Production of a viable variant of phage M13 with incorporated foreign peptide in the major coat protein. Doklady Akademii nauk SSSR., 307, 481-483. Doklady Biochemistry (Proc. Acad.  Sci.  USSR)-Engl.Tr., 1989, 307, 196-198.  

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