Valery A. Petrenko
A. Petrenko, Professor in the Department of Pathobiology at
Auburn University; graduated
Moscow State University,
U.S.S.R (1972); received PhD and D.Sc. degrees in chemistry from the
Zelinski Institute of Organic
Chemistry, (1976) and Moscow State University (1988), Moscow, U.S.S.R., Honor Ranks of Senior Scientist in Bioorganic Chemistry (1984) and Professor in Molecular Biology
(1992) from the Supreme Attestation Committee of the Government of the U.S.S.R. His Ph.D. research
focused on the synthesis of O-specific antigens of Salmonella, and D.Sc.—on
directed engineering of DNA and proteins. In
1977 he moved to Novosibirsk and worked as Junior and Senior Scientist
(1977-1982), Laboratory Head (1982-1985), Associate Director for Science (1985-1989), Director of Institute,
Associate General Director for Science and Professor (1989-1993) in
Scientific Association “Vector”
(Novosibirsk, Russia). In 1993 he joined the
faculty of University of
Missouri-Columbia as Visiting Professor and Research Professor, and in
2000—Auburn University as Professor.
He was appointed to the Graduate Faculty in 2001. In
Auburn Dr. Petrenko established a
research program focused on development of diagnostic and therapeutic probes using
phage display and phage nanobiotechnology. Dr. Petrenko is recipient (PI) of
grants from the Army Research
Office and the
Institute of Health,
Calvert Research LLC, recipient of the Pfizer Animal Health Award for
Research Excellence (2006, 2011) and CoPI in several collaborative grants. He is
member of American
Association of Pharmaceutical Scientists and Phi Zeta Honor Society of
Veterinary Medicine, included in
"Who's Who in Medicine and Healthcare", "Who's Who in America" 2011 (65th Edition),
is Who in the World" (15th Edition). and
"Who's Who in Science and Engineering" 2011-2012 (11th Edition). .
- Targeted drug/gene delivery
- Diagnosis of infectious diseases and cancer
- Monitoring and detection of biological threats
To attack these
problems, Professor Petrenko employs methods of phage display and phage nanobiotechnology
developed by his group.
The phage model is a gift from Dr.
Gregory P. Kishchenko.
2012. Wang, T.;
Petrenko, V.A.; Torchilin, V.P. Optimization of Landscape Phage Fusion
Protein-Modified Polymeric PEG-PE Micelles for Improved Breast Cancer Cell
Targeting. J Nanomedic Nanotechnol S4:008 (2012).
Huan Qi; Haiqin Lu; Huaji Qiu; Valery A Petrenko; Aihua Liu.Phagemid vectors for phage display: properties, characteristics and construction.
J. Mol. Biol. (2012), 417(3):129-43.
Olusegun A. Fagbohun, Deepa Bedi, Natalia I. Grabchenko,
Patricia A. Deinnocentes, Richard C. Bird, and Valery A. Petrenko. Landscape
phages and their fusion proteins targeted to breast cancer cells. Protein
Engineering, Design & Selection pp. 1–13, 2012.
2011. Phage Nanobiotechnology
(Eds. Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011)
Valery A. Petrenko and Prashanth K.
Jayanna, Phage-Mediated Drug Delivery. In: “Phage Nanobiotechnology” (Eds.
Valery Petrenko & George Smith), RSC Publishing, Cambridge, UK (2011).
Suiqiong Li, Ramji S. Lakshmanan, Valery A.
Petrenko and Bryan A. Chin. Chapter 6. Phage-Based Pathogen Biosensors. In:
“Phage Nanobiotechnology” (Eds. Valery Petrenko & George Smith), RSC Publishing,
Cambridge, UK (2011).
Wang T, Kulkarni N, D'Souza GG, Petrenko
VA, Torchilin VP. On the mechanism of targeting of phage fusion
protein-modified nanocarriers: only the binding Peptide sequence matters.
Mol Pharm. 2011 Oct 3;8(5):1720-8.
Wang T, Kulkarni N, Bedi D, D'Souza GG, Papahadjopoulos-Sternberg B,
Petrenko VA, Torchilin VP. In
vitro optimization of liposomal nanocarriers prepared from breast tumor cell
specific phage fusion protein.
J Drug Target. 2011
Bieke Van Dorst, Jaytry Mehta, Elsa Rouah-Martin, Wim De Coen, Valery Petrenko,
Ronny Blusta and Johan Robbens. Selection of PCB binding phages as potential
biorecognition elements for food and environmental monitoring.
Anal. Methods, 2011, 3, 1865.
Horikawa, S; Bedi, D; Li, SQ; Shen,
W; Huang, SC; Chen, IH; Chai, YT; Auad, ML; Bozack, MJ; Barbaree, JM; Petrenko,
VA; Chin, BA. Effects of surface functionalization on the surface phage coverage
and the subsequent performance of phage-immobilized magnetoelastic biosensors.
Biosensors and Bioelectronics, 2011 Jan 15;26(5):2361-7. Epub 2010 Oct 14.
Deepa Bedi, Tiziana Musacchio,
Olusegun A. Fagbohun, Patricia Deinnocentesa, R. Curtis Bird,
Lonnie Bookbinder, Vladimir
P. Torchilin, and Valery A. Petrenko. Delivery of siRNA into breast cancer cells
via phage fusion protein-targeted liposomes. Nanomedicine: Nanotechnology,
Biology, and Medicine,
315-323 JUN 2011.
2010 Abbineni, Gopal; Modali, Sita; Safiejko-Mroczka, Barbara;
Petrenko, Valery; Mao, Chuanbin. "Evolutionary selection of new breast
cancer cell-targeting peptides and phages with the cell-targeting peptides
fully displayed on the major coat and their effects on actin dynamics during
cell internalization". Mol Pharm. 2010,
VOL. 7, NO. 5, 1629–1642. PMID: 20735141
Wang T, Petrenko VA, Torchilin VP.
Polymeric Micelles Modified with MCF-7 Cell-Specific Phage Protein: Enhanced
Binding to Target Cancer Cells and Increased Cytotoxicity.
Pharm. 2010, 7(4):1007-14.PMID: 20518562
Wang T, D'Souza GG, Bedi D, Fagbohun OA,
Potturi LP, Papahadjopoulos-Sternberg B, Petrenko VA, Torchilin VP.
Enhanced binding and
killing of target tumor cells by drug-loaded liposomes modified with
tumor-specific phage fusion coat protein. Nanomedicine (Lond).
2010 Jun;5(4):563-74.PMID: 20528452
Wang T, Yang S, Petrenko VA, Torchilin VP.
of Liposomes into MCF-7 Breast Cancer Cells Mediated by Cell-Specific Phage
Fusion Coat Protein. Mol Pharm. 2010 Jun 14. [Epub ahead of print]PMID:
Jayanna PK, Bedi D, Deinnocentes P, Bird RC, Petrenko VA.
ligands for PC3 prostate carcinoma cells. Protein Eng Des Sel. 2010 Feb
25. [Epub ahead of print]PMID: 20185523
Jayanna PK, Bedi D, Gillespie JW, Deinnocentes P,
Wang T, Torchilin VP, Bird RC, Petrenko VA.
Landscape phage fusion
protein-mediated targeting of nanomedicines enhances their prostate tumor
cell association and cytotoxic efficiency. Nanomedicine. 2010 Feb
4. [Epub ahead of print]
Shen, W; Mathison, LC; Petrenko, VA;
Chin, BA. Design and characterization of a magnetoelastic sensor for the
detection of biological agents. Journal of Physics D-Applied Physics 43 (1):
Art. No. 015004 JAN 13 2010.
2009 G.A. Kuzmicheva, P.K. Jayanna, A.M.
Eroshkin, M.A. Grishina, E.S. Pereyaslavskaya, V.A. Potemkin, and V.A.
Mutations in fd phage major coat protein modulate affinity of the displayed
Protein Engineering, Design and Selection 2009 22: 631-639. PEDS Advance
Access published on July 25, 2009.
Jayanna P.K., Torchilin V.P. and V.A. Petrenko
(2009) Liposomes targeted by fusion phage proteins. Nanomedicine, 2009
Mar;5(1):83-9. Epub 2008 Oct 1.
Shen, W; Lakshmanan, RS; Mathison, LC; Petrenko, VA;
Chin, BA. Phage coated magnetoelastic micro-biosensors for real-time
detection of Bacillus anthracis spores. Sensors and Actuators B-Chemical 137
(2): 501-506 APR 2 2009.
S. Huang, H. Yang, R.S. Lakshmanan, M.L. Johnson, J. Wan, I.-H. Chen,
H.C. Wikle III, V.A. Petrenko, J.M. Barbaree, B.A. Chin. Sequential
detection of Salmonella typhimurium and Bacillus anthracis
sporesusing magnetoelastic biosensors. Biosensors and Bioelectronics.
24 (6), p.1730-1736, Feb 2009.
G.A. Kuzmicheva, P.K. Jayanna, I.B. Sorokulova
and V.A. Petrenko (2009) Diversity and censoring of landscape phage
libraries. Protein Engineering, Design, and Selection. 2009
Jan;22(1):9-18. Epub 2008 Nov 6.
2008 S. Huang, S.-Q. Li, H. Yang, M. Johnson, J.
Wan, I. Chen, V. A. Petrenko, J. M. Barbaree, and B. A. Chin. Optimization
of Phage-Based Magnetoelastic Biosensor Performance. Sensors & Transducers
Journal, Vol. 3, Special Issue, December 2008, pp.87-96.
(2008) Evolution of Phage
Display: From Bioactive Peptides to Bioselective Nanomaterials. Expert Opinion on Drug
Delivery (EODD), Aug;5(8):825-36.
S. Huang, H. Yang, R.S. Lakshmanan, M.L. Johnson, I. Chen, J. Wan, H.C.
Wikle, V.A. Petrenko, J.M. Barbaree, Z.Y. Cheng, B.A. Chin (2008). The
Effect of Salt and Phage Concentrations on the Binding Sensitivity of
Magnetoelastic Biosensors for Bacillus anthracis Detection. Biotechnology
and Bioengineering, 101 (5): 1014-1021 DEC 1 2008.
Johnson M.L., Wan
J., Huang S., Cheng Z., Petrenko V.A. Kim
D.-J., Chen I.-H., Barbaree J.M., Hong J.W., and B.A. Chin (2008). A
Wireless Biosensor Using Microfabricated Phage-Interfaced Magnetoelastic
Particles. Sensors & Actuators A: Physical. Vol 144/1 pp 38-47.
Brigati, J.R., Samoylova, T.I., Jayanna, P.K. and V.A.
Petrenko (2008) Phage display technique for generating
peptide reagents. In: Current Protocols in Protein Science (Editor Ben M.
Dunn, et al.). John Wiley & Sons.
(2008). Landscape phage as a molecular recognition interface for detection
devices. Review. Microelectronics Journal, Vol 39/2 pp 202-207.
Suiqiong Li, Michael Johnson, Jiehui Wan, Valery Petrenko, and Bryan Chin.
“Microfabricated Magnetoelastic Biosensors for the Detection of Bacillus
Anthracis Spores.” ECS Transactions (Issue Title: Chemical Sensors 8:
Chemical (Gas, Ion, Bio) Sensors and Analytical Systems), Vol. 16, pp
177-185, 2008. doi:10.1149/1.2981119
2007 Fu. L., Li, S., Zhang, K., Chen, I-H., Petrenko, V.A.
and Z.-Y. Cheng, (2007), Magnetostrictive microcantilever as an advanced
transducer for biosensors, Sensors Journal, V.7, 2929-2941.
Lakshmanan, RS; Guntupalli, R; Hu, J; Petrenko,
VA; Barbaree, JM; Chin, BA. Detection of Salmonella typhimurium in
fat free milk using a phage immobilized magnetoelastic sensor. Sensors and
actuators B-Chemical 126 (2): 544-550 OCT 1 2007
Lakshmanan RS, Guntupalli R, Hu J, Kim DJ, Petrenko VA, Barbaree JM,
Chin BA (2007). Phage based magnetoelastic sensor for the detection of
Salmonella typhimurium. Microbiol Methods. Oct;71(1):55-60. Epub 2007 Aug 9.
Petrenko (2007) Phage-Derived Bioselective Nanovehicles For Drug And Gene
Delivery. Nanotech 2007, V. 2.
p.703-706, Technical Proceedings, CRC Press, Taylor & Francis Group.
Wan, J., Johnson, M., Guntupalli,R.,
Petrenko,V.A. and B.A. Chin (2007), "Detection of Bacillus anthracis spores in liquid using phage-based magnetoelastic micro-resonators", Sensors
and actuators B, 127: 559-566.
Shu, H., Huang, S., Fiebor, B., Chen, I-H., Petrenko, V.A., Chin, B.A.
(2007), Phage-based Magnetoelastic wireless biosensors for detecting
Bacillus anthracis spores. IEEE Sensor Journal, vol 7 No. 3 pp 470-477.
Petrenko V.A. & J.R. Brigati (2007) Phage As Biospecific Probes. In:
Immunoassay and other Bioanalytical Techniques (Editor Jeanette M. Van Emon). CRC
Press, Taylor & Francis Group, Boca Raton, FL, U.S.A..
Nanduri V., Sorokulova I.B.,
Samoylov A.M., Simonian A.L., Petrenko V.A., and V.J. Vodyanoy (2007).
Phage as molecular recognition elements in biosensors immobilized by
physical adsorption. Biosensors and Bioelectronics. 22(6):986-92..
E.Olsen, J. Sykora, I. Sorokulova, W. Neely, I. Chen, J. Barbaree V. Petrenko and V.
Vodyanoy (2007). Phage Fusion Proteins as Bioselective Receptors for Piezoelectric
Sensors. ECS Transactions, 2(19), 9-25.
Olsen E.V., Sorokulova I.B.,
Petrenko V.A., Chen I-H., Barbaree J.M., and V.J. Vodyanoy (2006).
bacteriophage as a probe for acoustic wave biodetectors of Salmonella
typhimurium. Biosensors and
Bioelectronics. V21: p.1434-1442.
Petrenko V.A. & G.P. Smith (2005) Vectors and Modes of Display. In: Phage Display in Biotechnology and Drug Discovery (Editor S. Sidhu).
CRC Press, Taylor & Francis Group, Boca Raton, FL, U.S.A., 714pp.
Brigati J.R. and V.A. Petrenko
(2005). Thermostability of Landscape Phage Probes. Analytical and
Bioanalytical Chemistry. V.382, p.1346-1350.
Sorokulova I.B., E.V.
Olsen, I-H. Chen, B. Fiebor, J.M. Barbaree, V.J. Vodyanoy, B.A. Chin and V.A.
Petrenko (2005). Landscape Phage Probes for Salmonella
typhimurium. The Journal of
Microbiological Methods. V. 63, No 1, p. 55-72..
2004 Mount J., T.I. Samoylova, N.E. Morrison, N.R. Cox, H.J. Baker
V.A. Petrenko. (2004) Cell Targeted Phagemid Recurred by Pre-Selected
Landscape Phage. Gene. V.341, p.59-65.
Brigati J., D.D. Williams, I.B.
Sorokulova, V. Nanduri, I-H. Chen, C.L. Turnbough, Jr. and V.A. Petrenko
Diagnostic probes for Bacillus anthracis
spores selected from a landscape phage library. Clinical Chemistry.
V.50, No.11, p.1899-1906.
Samoylova T.I. , N.R. Cox, N.E. Morrison, L.P. Globa, V.
Romanov, H.J. Baker, and V.A. Petrenko (2004). Phage matrix for
isolation of glioma cell-membrane proteins. BioTechniques, V.37,
No. 2, p.254-260.
Petrenko V.A. and I.B. Sorokulova
(2004) Detection of Biological Threat Agents. A challenge for
Combinatorial Biochemistry. The Journal of Microbiological Methods Vol 58/2
Samoylova T.I., V.A. Petrenko, N.E. Morrison, L.P. Globa, H.J.
Baker, and N.R. Cox (2003). Phage Probes for Malignant Glial Cells. Molecular Cancer Therapeutic. V.2, p.1129-1137.
V.A. and V.J.
Vodyanoy (2003) Phage display for detection of biological threat agents.
The Journal of
53/2 pp. 243-252.
V.A., Smith G.P., Mazooji M.M. and Quinn T.
(2002) Alfa-helically constrained phage display
library. Protein Engineering, V.15, No.11, pp.943-950.
Romanov V.I., Durand D.E. and Petrenko V.A.
(2001) Phage Display
Selection On Prostate Carcinoma Cells Yields A Peptide Affecting Cells Spreading
And Invasion. The Prostate, V.47, p.239-251.
Kouzmitcheva G.A., Petrenko V.A. and Smith G.P.
Peptides for Lyme Disease Through Epitope Discovery. Clinical and Diagnostic
Laboratory Immunology, V8, p.150-160.
V.A. and Smith G.P. (2000) Phage from landscape libraries as substitute antibodies.
Protein Engineering, V.13, N8, p.101-104.
Smith G.P., V.A. Petrenko and L.L. Metthews (1998) Cross-Linked
Filamentous Phage as an Affinity Matrix. Journal of Immunological Methods,
Smith G.P. and Petrenko V.A.
(1997) Phage Display.
Reviews, V.97, N2, p. 391-410.
V.A., Smith G.P.,
Gong X. and Quinn T. (1996) A library of organic landscapes on filamentous
phage. Protein Engineering, V.9, N9, p. 797-801.
Minenkova O.O., A.A. Ilyichev,
G.P. Kishchenko and V.A. Petrenko (1993) Design of specific immunogen
using filamentous phages as a carrier. Gene, 128, 85-88.
Kishchenko G.P., O.O. Minenkova, A.A. Ilyichev, A.D. Gruzdev, V.A. Petrenko (1991) Study of the structure of phage-M13 virions containing chimeric B-protein molecules. Molekulyarnaya biologiya,
25, 1497-1503. Molecular Biology, 1991, 25(6), 1171-1176.
Ilyichev A.A., O.O. Minenkova,
S.I. Tatkov, N.N. Karpyshev, A.M. Eroshkin, V.A. Petrenko, L.S.
Sandakhchiev (1989) Production of a viable variant of phage M13 with
incorporated foreign peptide in the major coat protein. Doklady Akademii
nauk SSSR., 307, 481-483.
Doklady Biochemistry (Proc. Acad. Sci.
USSR)-Engl.Tr., 1989, 307, 196-198.